human monocytic leukemia cell line  (ATCC)

Journal: Frontiers in Immunology

Article Title: The long noncoding RNA lnc-FAM164A1 -ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach

doi: 10.3389/fimmu.2026.1776849

Figure Lengend Snippet: Enforced expression of lnc-FAM164A1 promotes the activation of NF-κB signaling pathway in macrophages. (A) Enforced expression of lnc-FAM164A1 enhances the activation of NF-κB promoter assessed by NF-κB-luciferase reporter assay in HEK293 cells ( P < 0.05 Vector vs Lnc-FAM164A1 , n = 9 cell culture replicates). (B) Enforced expression of lnc-FAM164A1 by adenovirus enhances the degradation of IκB-α in THP-1 macrophages; and (C) in human PBMC-derived macrophages up to 60 minutes of LPS induction. (D) Enforced expression of lnc-FAM164A1 also increased the nuclear accumulation of p65 in human PBMC-derived macrophages up to 60 minutes of LPS induction. IκB-α and p65 protein in nuclear extracts were detected by Western blots. β-actin and laminA/C were used as loading controls.

Article Snippet: The human monocytic leukemia cell line (THP-1) was purchased from ATCC (TIB-202) and cultured with Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin in cell culture incubator at 37°C (5% CO2).

Techniques: Expressing, Activation Assay, Luciferase, Reporter Assay, Plasmid Preparation, Cell Culture, Derivative Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: The long noncoding RNA lnc-FAM164A1 -ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach

doi: 10.3389/fimmu.2026.1776849

Figure Lengend Snippet: RNA immunoprecipitation (RIP) demonstrates the interaction between ACLY and lnc-FAM164A1 . (A) Western blot of a representative immunoprecipitation of ACLY protein from THP-1 differentiated macrophages stimulated with 10 ng/mL for 3 hours. ACLY signal in input (before immunoprecipitation), flow-through (FT), washes, and IP sample. (B) Lnc-FAM164A1 levels from immunoprecipitations after 2-, 3- and 6-hours stimulation with LPS was measured by qPCR (P<0.05, IgG control vs ACLY, n = 3 independent experiments). (C) the sequence of ACLY, full-length lnc-FAM164A1 and lnc-FAM164A1 with partial deletion were submitted into lncPro. (D) Proposed working model of the lnc-FAM164A1 –ACLY–NF-κB axis in LPS-activated macrophages. Created in BioRender.com .

Article Snippet: The human monocytic leukemia cell line (THP-1) was purchased from ATCC (TIB-202) and cultured with Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin in cell culture incubator at 37°C (5% CO2).

Techniques: RNA Immunoprecipitation, Western Blot, Immunoprecipitation, Control, Sequencing